Composite

Part:BBa_K1132036

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-19)

XOR gate (BBa_K1132004) with RFP

DO NOT USE this part! The FimE sites did not work well, this design can be use by replace K137008 by K1077001 and K137010 by K1077000.

This BioBrick is design to test the XOR gate (BBa_K1132004) by measuring the level of RFP after recombination events. The biobrick BBa_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites.

2036.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 178
    Illegal AgeI site found at 290
  • 1000
    COMPATIBLE WITH RFC[1000]


See BBa_K1132038 for characterization.

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Categories
Parameters
None